HLA-B27 Determination by Polymerase Chain Reaction | Zendy

D. C. Kilpatrick | Zendy

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HLA-B27 Determination by Polymerase Chain Reaction

Author(s) -

D. C. Kilpatrick

Publication year - 1996

Publication title -

disease markers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.912

H-Index - 66

eISSN - 1875-8630

pISSN - 0278-0240

DOI - 10.1155/1996/184375

Subject(s) - polymerase chain reaction , primer (cosmetics) , typing , microbiology and biotechnology , human leukocyte antigen , exon , hla b27 , biology , hla b , polymerase , genetics , serology , gene , virology , chemistry , antigen , antibody , organic chemistry

A method for determining the presence or absence of HLA-B27 by selective amplification of a region in the third exon of the HLA-B27 gene common to B*2701 to B*2705 inclusive, was evaluated. This polymerase chain reaction/sequence specific primer (PCR-SSP) method gave perfect correlation with serological typing on 40 individuals of previously determined HLA type and on 50 further clinical samples evaluated blind. It was concluded that HLA-B27 determination by PCR-SSP is simple, reliable, cost-effective and convenient for laboratory staff.

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