Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

Mostof the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacologicalactivity or yield antibodies which have lost much of their immunological specificity. The use ofglutaraldehyde for coupling saves both the pharmacological and immunological activities but it causessome polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use.A new coupling procedure is reported which uses an activated derivative of daunorubicin added tomonoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significantpolymerization of the antibody or Joss of pharmacological activity determined by testing normal and tumourcell Lines in vitro