CORP: The assessment of total hemoglobin mass by carbon monoxide rebreathing | Zendy

Christoph Siebenmann | Zendy; Stefanie Keiser | Zendy; Paul Robach | Zendy; Carsten Lundby | Zendy

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CORP: The assessment of total hemoglobin mass by carbon monoxide rebreathing

Author(s) -

Christoph Siebenmann,

Stefanie Keiser,

Paul Robach,

Carsten Lundby

Publication year - 2017

Publication title -

journal of applied physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.253

H-Index - 229

eISSN - 8750-7587

pISSN - 1522-1601

DOI - 10.1152/japplphysiol.00185.2017

Subject(s) - carboxyhemoglobin , hemoglobin , carbon monoxide , chemistry , dilution , reproducibility , chromatography , anesthesia , medicine , biochemistry , thermodynamics , physics , catalysis

In this Cores of Reproducibility in Physiology (CORP) article, we present the theory and practical aspects of the carbon monoxide (CO) rebreathing method for the determination of total hemoglobin mass in humans. With CO rebreathing, a small quantity of CO is diluted in O 2 and rebreathed for a specified time period, during which most of the CO is absorbed and bound to circulating hemoglobin. The dilution principle then allows calculation of the total number of circulating hemoglobin molecules based on the number of absorbed CO molecules and the resulting changes in the fraction of carboxyhemoglobin in blood. Total hemoglobin mass is derived by multiplication with the molar weight of hemoglobin. CO rebreathing has been used for >100 yr and has undergone steady improvement so that today excellent values in terms of accuracy and precision can be achieved if the methodological precautions are carefully followed.

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