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The aim of this study was to investigate the later effects of daily [Formula: see text] supplementation over 3 wk of training on the relationship between O 2  uptake and power at different intensities with an incremental test (IT), a double-wingate test (WT), and an endurance capacity test at 80% W max  (ECT) before and after the supplementation period. Seventeen male recreational athletes participated in this double-blind placebo (PL)-controlled study. Subjects participated in a 3-wk intermittent high-intensity, high-volume training period with 45 intervals of W max  − 10 W and an active recovery period of 10 W in between with dietary [Formula: see text] (NaNO 3 ) or placebo supplementation (NaCl) (both 8.5 mg·kg −1 ·day −1 ) on a cycle ergometer. During a training session, plasma [[Formula: see text]] ( P &lt; 0.001) and plasma [[Formula: see text]] ( P &lt; 0.01) were higher in nitrate (N), whereas in pre- and posttests mean plasma [[Formula: see text]] and [[Formula: see text]] were not different between groups. In the WT [48 h after cessation of supplementation (C)], the ratio between V̇o 2  and power decreased in N ( P &lt; 0.01) with no changes in PL. Endurance capacity (4–5 days after C) similarly increased in both groups ( P &lt; 0.01). However, the total oxygen consumption decreased by 5% ( P &lt; 0.01) in N, with no change in PL. The slope of V̇o 2 ·W −1  in IT (5–7 days after C) decreased in N ( P &lt; 0.01), whereas no changes were found in PL. During low- and moderate-intensity workloads, no changes and differences in V̇o 2  could be detected. We conclude that nitrate supplementation causes a sustaining reduction of the oxygen cost per watt during exercise with a large recruitment of type II muscle fibers without affecting endurance capacity.    NEW &amp; NOTEWORTHY Because most studies focused on the acute effects of [Formula: see text] supplementation on exercise performance during a supplementation period, the sustainability of the effects of the [Formula: see text] supplementation remain unknown. We followed the development of V̇o 2 /W at different intensities during the first week after cessation of daily [Formula: see text] supplementation over 3 wk. The results indicate that [Formula: see text] supplementation has a long-term effect for at least 7 days after cessation during heavy all-out workloads without affecting endurance capacity.

Long-term effects of NO3– on the relationship between oxygen uptake and power after three weeks of supplemented HIHVT