Open AccessT cell transfer model of chronic colitis: concepts, considerations, and tricks of the trade
Author(s) -
Dmitry V. Ostanin,
Jianxiong Bao,
Iurii Koboziev,
Laura Gray,
Sherry A. Robinson-Jackson,
Melissa Kosloski-Davidson,
V. H. Price,
Matthew B. Grisham
Publication year - 2008
Publication title -
ajp gastrointestinal and liver physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 169
eISSN - 1522-1547
pISSN - 0193-1857
DOI - 10.1152/ajpgi.90462.2008
Subject(s) - pathogenesis , inflammatory bowel disease , colitis , ulcerative colitis , inflammation , immunology , medicine , disease , animal model , pathology
The inflammatory bowel diseases (Crohn's disease; ulcerative colitis) are idiopathic chronic inflammatory disorders of the intestine and/or colon. A major advancement in our understanding of the pathogenesis of these diseases has been the development of mouse models of chronic gut inflammation. One model that has been instrumental in delineating the immunological mechanisms responsible for the induction as well as regulation of intestinal inflammation is the T cell transfer model of chronic colitis. This paper presents a detailed protocol describing the methods used to induce chronic colitis in mice. Special attention is given to the immunological concepts that explain disease pathogenesis in this model, considerations and potential pitfalls in using this model, and finally different "tricks" that we have learned over the past 12 years that have allowed us to develop a more simplified version of this model of experimental IBD.
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