Distinct miRNA profiles in normal and gastric cancer myofibroblasts and significance in Wnt signaling | Zendy

Liyi Wang | Zendy; Islay Steele | Zendy; J. Dinesh Kumar | Zendy; R. Dimaline | Zendy; Puthen V. Jithesh | Zendy; László Tiszlavicz | Zendy; Zita Reisz | Zendy; Graham J. Dockray | Zendy; Andrea Varró | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Distinct miRNA profiles in normal and gastric cancer myofibroblasts and significance in Wnt signaling

Author(s) -

Liyi Wang,

Islay Steele,

J. Dinesh Kumar,

R. Dimaline,

Puthen V. Jithesh,

László Tiszlavicz,

Zita Reisz,

Graham J. Dockray,

Andrea Varró

Publication year - 2016

Publication title -

ajp gastrointestinal and liver physiology

Language(s) - English

Resource type - Journals

eISSN - 1522-1547

pISSN - 0193-1857

DOI - 10.1152/ajpgi.00443.2015

Subject(s) - wnt signaling pathway , stromal cell , microrna , cancer research , biology , downregulation and upregulation , microbiology and biotechnology , gene knockdown , cell migration , microarray analysis techniques , extracellular matrix , chemokine , myofibroblast , cancer cell , cancer , signal transduction , cell , immunology , fibrosis , pathology , cell culture , gene expression , medicine , inflammation , gene , biochemistry , genetics

Stromal cells influence epithelial function in both health and disease. Myofibroblasts are abundant stromal cells that influence the cellular microenvironment by release of extracellular matrix (ECM) proteins, growth factors, proteases, cytokines, and chemokines. Cancer-associated myofibroblasts (CAMs) differ from adjacent tissue (ATMs) and normal tissue myofibroblasts (NTMs), but the basis of this is incompletely understood. We report now the differential expression of miRNAs in gastric cancer CAMs. MicroRNA arrays identified differences in the miRNA profile in gastric and esophageal NTMs and in CAMs from stomach compared with NTMs. miR-181d was upregulated in gastric CAMs. Analysis of differentially regulated miRNAs indicated an involvement in Wnt signaling. Examination of a microarray data set then identified Wnt5a as the only consistently upregulated Wnt ligand in gastric CAMs. Wnt5a stimulated miR-181d expression, and knockdown of miR-181d inhibited Wnt5a stimulation of CAM proliferation and migration. Analysis of miR-181d targets suggested a role in chemotaxis. Conditioned medium from CAMs stimulated gastric cancer cell (AGS) migration more than that from ATMs, and miR-181d knockdown reduced the effect of CAM-CM on AGS cell migration but had no effect on AGS cell responses to ATM conditioned media. The data suggest that dysregulation of miRNA expression in gastric CAMs, secondary to Wnt5a signaling, accounts at least in part for the effect of CAMs in promoting cancer cell migration.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research