Electrical stimulation facilitates the angiogenesis of human umbilical vein endothelial cells through MAPK/ERK signaling pathway by stimulating FGF2 secretion | Zendy

Kang Geng | Zendy; Jing Wang | Zendy; Pengfei Liu | Zendy; Xinli Tian | Zendy; Hongjun Liu | Zendy; Xue Wang | Zendy; Chunbing Hu | Zendy; Hong Yan | Zendy

Open Access

Electrical stimulation facilitates the angiogenesis of human umbilical vein endothelial cells through MAPK/ERK signaling pathway by stimulating FGF2 secretion

Author(s) -

Kang Geng,

Jing Wang,

Pengfei Liu,

Xinli Tian,

Hongjun Liu,

Xue Wang,

Chunbing Hu,

Hong Yan

Publication year - 2019

Publication title -

ajp cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.432

H-Index - 181

eISSN - 1522-1563

pISSN - 0363-6143

DOI - 10.1152/ajpcell.00474.2018

Subject(s) - mapk/erk pathway , angiogenesis , umbilical vein , fibroblast growth factor , secretion , signal transduction , microbiology and biotechnology , vascular endothelial growth factor , protein kinase a , vascular endothelial growth factor a , human umbilical vein endothelial cell , kinase , cancer research , biology , chemistry , endocrinology , medicine , in vitro , biochemistry , receptor , vegf receptors

Electrical stimulation (ES) is able to enhance angiogenesis by stimulating fibroblasts. Fibroblast growth factor 2 (FGF2) is an independent angiogenesis inducer. The present study aimed to evaluate the role of ES-induced FGF2 secretion in affecting angiogenesis during wound healing via the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway. Fibroblasts and human umbilical vein endothelial cells (HUVECs) were exposed to ES, and the HUVECs were cocultured with ES-treated fibroblast culture solution. ES exposure showed no toxic effects on fibroblasts or HUVECs. ES led to enhanced growth of fibroblasts and HUVECs as well as FGF2 secretion, which is induced through the NOS pathway. ES-induced FGF2 secretion was shown to increase vascular endothelial growth factor (VEGF) protein and enhance migration, invasion, and angiogenesis of HUVECs. Also, ES-induced FGF2 secretion activated the MAPK/ERK signaling pathway. However, inhibition of the MAPK/ERK signaling pathway reversed the positive effects of ES-induced FGF2 secretion. In vitro experiments showed positive effects of ES on wound healing. Taken together, the findings suggested that ES promoted FGF2 secretion and then activated the MAPK/ERK signaling pathway by facilitating angiogenesis and promoting wound healing.

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