Intravital FRET: comprehending life at single-molecule level. Focus on “A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy” | Zendy

Randall L. Lindquist | Zendy; Raluca Niesner | Zendy

Open Access

Intravital FRET: comprehending life at single-molecule level. Focus on “A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy”

Author(s) -

Randall L. Lindquist,

Raluca Niesner

Publication year - 2015

Publication title -

ajp cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.432

H-Index - 181

eISSN - 1522-1563

pISSN - 0363-6143

DOI - 10.1152/ajpcell.00286.2015

Subject(s) - förster resonance energy transfer , two photon excitation microscopy , fluorescence microscope , microscopy , biophysics , nanotechnology , fluorescence , physics , materials science , biology , optics

"A practical Method for Monitoring FRET-based Biosensors in Living Animals Using Two-photon Microscopy" by Richard Day and colleagues throughly discusses the main issues regarding the use of FRET, as a tool to monitor cellular and tissue function, in vivo and, at the same time, provides an excellent road map for calibrating FRET biosensors in living animals to scientists in diverse bioscientifical research areas.

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