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"A practical Method for Monitoring FRET-based Biosensors in Living Animals Using Two-photon Microscopy" by Richard Day and colleagues throughly discusses the main issues regarding the use of FRET, as a tool to monitor cellular and tissue function, in vivo and, at the same time, provides an excellent road map for calibrating FRET biosensors in living animals to scientists in diverse bioscientifical research areas.

american journal of physiology. cell physiology

<i>Intravital FRET: comprehending life at single-molecule level</i>. Focus on “A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy”

Intravital FRET: comprehending life at single-molecule level. Focus on “A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy”