English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Diaphragm abnormalities in aging or chronic diseases include impaired mitochondrial respiration and H 2 O 2 emission, which can be measured using saponin-permeabilized muscle fibers. Mouse diaphragm presents a challenge for isolation of fibers due to relatively high abundance of connective tissue in healthy muscle that is exacerbated in disease states. We tested a new approach to process mouse diaphragm for assessment of intact mitochondria respiration and ROS emission in saponin-permeabilized fibers. We used the red gastrocnemius (RG) as “standard” limb muscle. Markers of mitochondrial content were two– to fourfold higher in diaphragm (Dia) than in RG ( P &lt; 0.05). Maximal O 2 consumption ( JO 2 : pmol·s −1 ·mg −1 ) in Dia was higher with glutamate, malate, and succinate (Dia 399 ± 127, RG 148 ± 60; P &lt; 0.05) and palmitoyl-CoA + carnitine (Dia 15 ± 5, RG 7 ± 1; P &lt; 0.05) than in RG, but not different between muscles when JO 2 was normalized to citrate synthase activity. Absolute JO 2 for Dia was two– to fourfold higher than reported in previous studies. Mitochondrial JH 2 O 2 was higher in Dia than in RG ( P &lt; 0.05), but lower in Dia than in RG when JH 2 O 2 was normalized to citrate synthase activity. Our findings are consistent with an optimized diaphragm preparation for assessment of intact mitochondria in permeabilized fiber bundles. The data also suggest that higher mitochondrial content potentially makes the diaphragm more susceptible to “mitochondrial onset” myopathy. Overall, the new approach will facilitate testing and understanding of diaphragm mitochondrial function in mouse models that are used to advance biomedical research and human health.

american journal of physiology. cell physiology

Mitochondrial respiration and H<sub>2</sub>O<sub>2</sub>emission in saponin-permeabilized murine diaphragm fibers: optimization of fiber separation and comparison to limb muscle

Mitochondrial respiration and H2O2emission in saponin-permeabilized murine diaphragm fibers: optimization of fiber separation and comparison to limb muscle

Mitochondrial respiration and H₂O₂emission in saponin-permeabilized murine diaphragm fibers: optimization of fiber separation and comparison to limb muscle