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ca2+ ion serves as a multifunctional messenger that is responsible for numerous cellular functions. Since the first recording of Ca2+ transients using aequorin in live cells, technological advances have been made on many fronts for monitoring intracellular Ca2+ dynamics. These advances include both the engineering of a large repertoire of Ca2+ fluorescent probes and target-specific biosensors whose optical properties are sensitive to intracellular Ca2+ concentration, as well as improvements in the detection and imaging of Ca2+ signals with high temporal and spatial resolution at subcellular levels within the cytosol and specific organelles such as sarcoplasmic reticulum, mitochondria, and nucleus.

american journal of physiology. cell physiology

Tracking stars: automated two-dimensional analysis of Ca2+ events. Focus on “Automated region of interest analysis of dynamic Ca2+ signals in image sequences”