English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Airway smooth muscle expresses abundant BK Ca channels, but their role in regulating contractions remains controversial. This study examines the effects of two potent BK Ca channel openers on agonist-induced phasic contractions in rabbit and mouse bronchi. First, we demonstrated the ability of 10 μM GoSlo-SR5-130 to activate BK Ca channels in inside-out patches from rabbit bronchial myocytes, where it shifted the activation V 1/2 by −88 ± 11 mV (100 nM Ca 2+ , n = 7). In mouse airway smooth muscle cells, GoSlo-SR5-130 dose dependently shifted V 1/2 by 12–83 mV over a concentration range of 1–30 μM. Compound X, a racemic mixture of two enantiomers, reported to be potent BK Ca channel openers, shifted V 1/2 by 20–79 mV over a concentration range of 0.3–3 μM. In rabbit bronchial rings, exposure to histamine (1 μM) induced phasic contractions after a delay of ~35 min. These were abolished by GoSlo-SR5-130 (30 μM). Nifedipine (100 nM) and CaCC inh A01 (10 μM), a TMEM16A blocker, also abolished histamine-induced phasic contractions. In mouse bronchi, similar phasic contractions were evoked by exposure to U46619 (100 nM) and carbachol (100 nM). In each case, these were inhibited by concentrations of GoSlo-SR5-130 and compound X that shifted the activation V 1/2 of BK Ca channels in the order of −80 mV. In conclusion, membrane potential-dependent regulation of L-type Ca 2+ channels appears to be important for histamine-, U46619-, and carbachol-induced phasic contractions in airway smooth muscle. Contractions can be abolished by BK Ca channel openers, suggesting that these channels are potential targets for treating some causes of airway obstruction.

american journal of physiology. cell physiology

Inhibitory effects of openers of large-conductance Ca<sup>2+</sup>-activated K<sup>+</sup>channels on agonist-induced phasic contractions in rabbit and mouse bronchial smooth muscle

Inhibitory effects of openers of large-conductance Ca2+-activated K+channels on agonist-induced phasic contractions in rabbit and mouse bronchial smooth muscle

Inhibitory effects of openers of large-conductance Ca²⁺-activated K⁺channels on agonist-induced phasic contractions in rabbit and mouse bronchial smooth muscle