Communication—Microfluidic Electrochemical Acetylcholine Detection in the Presence of Chlorpyrifos | Zendy

Ethan S. McClain | Zendy; Dusty R. Miller | Zendy; David E. Cliffel | Zendy

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Communication—Microfluidic Electrochemical Acetylcholine Detection in the Presence of Chlorpyrifos

Author(s) -

Ethan S. McClain,

Dusty R. Miller,

David E. Cliffel

Publication year - 2019

Publication title -

journal of the electrochemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.258

H-Index - 271

eISSN - 1945-7111

pISSN - 0013-4651

DOI - 10.1149/2.0711916jes

Subject(s) - chlorpyrifos , oxon , acetylcholine , chemistry , detection limit , acetylcholinesterase , amperometry , metabolite , cholinesterase , chromatography , biochemistry , pharmacology , enzyme , pesticide , electrochemistry , biology , electrode , neuroscience , agronomy

An amperometric acetylcholine sensor was developed for use in a microfluidic system and characterized with chlorpyrifos and its metabolite, chlorpyrifos oxon. This enzymatic sensor was highly selective for acetylcholine, with a detection limit of 0.2 μM and sensitivity of 1.7 nA μM −1 from 1–150 μM. Though chlorpyrifos had no effect on sensor function, chlorpyrifos oxon significantly inhibited response across a range of concentrations (0.5–50 μM). Inhibition was reversed by 2-pyridine aldoxime methyl chloride. This platform can be used to both quantify acetylcholine in the presence of chlorpyrifos and as a biorecognition method for chlorpyrifos oxon.

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