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This focus issue is devoted to Micro-Nano Systems in Health Care and Environmental Monitoring. It has been an exciting opportunity to collect together papers from invited speakers and authors who participated in the related symposium held at the 227th ECS meeting in Chicago in May 2015. This meeting brought together medical professionals, clinicians, engineers, chemists, biologists and physicists under the same roof. This symposium and the papers published in the focus issue provide for a synopsis of the research, development, and technological evolution of micro-nano sensors and systems in healthcare and environmental monitoring applications.