Open AccessQUANTIFYING THE SHORT LIFETIME WITH TCSPC-FLIM: FIRST MOMENT VERSUS FITTING METHODS
Author(s) -
Lingling Xu,
Zichen Wei,
Shaoqun Zeng,
ZhenLi Huang
Publication year - 2013
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545813500302
Subject(s) - fluorescence lifetime imaging microscopy , fluorescence , photon counting , optics , materials science , photon , combing , moment (physics) , microscopy , nuclear magnetic resonance , physics , classical mechanics , composite material
Combing the time-correlated single photon counting (TCSPC) with fluorescence lifetime imaging microscopy (FLIM) provides promising opportunities in revealing important information on the microenvironment of cells and tissues, but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique. Here we present a comprehensive investigation on the performance of two data analysis methods, the first moment (M1) method and the conventional least squares (Fitting) method, in quantifying fluorescence lifetime. We found that the M1 method is more superior than the Fitting method when the lifetime is short (70 ~ 400 ps) or the signal intensity is weak (<103 photons)