Open AccessNONLINEAR SPECTRAL IMAGING OF ELASTIC CARTILAGE IN RABBIT EARS
Author(s) -
Jing Chen,
Chungen Guo,
Hongsheng Li,
Xiaodong Zhu,
Shuyuan Xiong,
Jianxin Chen
Publication year - 2013
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545813500247
Subject(s) - cartilage , nicotinamide adenine dinucleotide , flavin adenine dinucleotide , materials science , biophysics , chemistry , biomedical engineering , anatomy , biology , biochemistry , cofactor , nad+ kinase , medicine , enzyme
Elastic cartilage in the rabbit external ear is an important animal model with attractive potential value for researching the physiological and pathological states of cartilages especially during wound healing. In this work, nonlinear optical microscopy based on two-photon excited fluorescence and second harmonic generation were employed for imaging and quantifying the intact elastic cartilage. The morphology and distribution of main components in elastic cartilage including cartilage cells, collagen and elastic fibers were clearly observed from the high-resolution two-dimensional nonlinear optical images. The areas of cell nuclei, a parameter related to the pathological changes of normal or abnormal elastic cartilage, can be easily quantified. Moreover, the three-dimensional structure of chondrocytes and matrix were displayed by constructing three-dimensional image of cartilage tissue. At last, the emission spectra from cartilage were obtained and analyzed. We found that the different ratio of collagen over elastic fibers can be used to locate the observed position in the elastic cartilage. The redox ratio based on the ratio of nicotinamide adenine dinucleotide (NADH) over flavin adenine dinucleotide (FAD) fluorescence can also be calculated to analyze the metabolic state of chondrocytes in different regions. Our results demonstrated that this technique has the potential to provide more accurate and comprehensive information for the physiological states of elastic cartilage