Open AccessPostmortem survival characteristics of rat glial cells in culture.
Author(s) -
Peter G. E. Kennedy
Publication year - 1987
Publication title -
journal of neurology neurosurgery and psychiatry
Language(s) - English
Resource type - Journals
eISSN - 1468-330X
pISSN - 0022-3050
DOI - 10.1136/jnnp.50.6.798
Subject(s) - glial fibrillary acidic protein , galactocerebroside , biology , gfap stain , in vitro , programmed cell death , neuroglia , astrocyte , immunofluorescence , cell culture , pathology , microbiology and biotechnology , oligodendrocyte , andrology , immunology , immunohistochemistry , central nervous system , antibody , medicine , neuroscience , apoptosis , biochemistry , genetics , myelin
Cell-type-specific markers and indirect immunofluorescence were used to determine the longest time interval between death of the animal and removal of tissues that permitted growth in vitro of rat central glial cells. Galactocerebroside+ oligodendrocytes could not be cultured after more than 1 hour had elapsed, whereas small numbers of glial fibrillary acidic protein (GFAP)+ astrocytes and GFAP- fibroblastic cells could still be cultured for as long as 6-7 hours after death. These observations may reflect a greater susceptibility of oligodendrocytes to anoxia compared with astrocytes and fibroblasts.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom