Diagnosis of childhood BK virus cystitis by electron microscopy and PCR. | Zendy

Kazuhito Saitoh | Zendy; N Sugae | Zendy; N Koike | Zendy; Yutaka Akiyama | Zendy; Y. Iwamura | Zendy; Hiroshi Kimura | Zendy

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Diagnosis of childhood BK virus cystitis by electron microscopy and PCR.

Author(s) -

Kazuhito Saitoh,

N Sugae,

N Koike,

Yutaka Akiyama,

Y. Iwamura,

Hiroshi Kimura

Publication year - 1993

Publication title -

journal of clinical pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.1

H-Index - 127

eISSN - 1472-4146

pISSN - 0021-9746

DOI - 10.1136/jcp.46.8.773

Subject(s) - bk virus , urinary sediment , virus , polyomavirus infections , papanicolaou stain , polymerase chain reaction , pathology , jc virus , hemorrhagic cystitis , virology , medicine , urinary bladder , urinary system , biology , kidney , urology , gene , cancer , disease , cervical cancer , biochemistry , progressive multifocal leukoencephalopathy , kidney transplantation , hematopoietic stem cell transplantation

A case of BK virus cystitis in a 5 year old boy is reported. This patient, who was not immunocompromised, had had acute cystitis for two weeks. Many intracytoplasmic inclusions were observed in urinary sediment smears stained by the Papanicolaou method. Electron microscopic examination showed virus particles, presumed to be human polyomavirus, in the nuclei of the degenerated urothelial cells. A DNA sequence of the BK virus was detected in 200-300 urothelial cells in Papanicolaou stained smears by the polymerase chain reaction. BK virus is an unusual cause of symptomatic cystitis in a healthy child.

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