New tool for the simultaneous detection of 10 different genotypes of Acanthamoeba available from the American Type Culture Collection | Zendy

P. Goldschmidt | Zendy; Sandrine Degorge | Zendy; Djida Ghoubay | Zendy; C Saint-Jean | Zendy; L. Batellier | Zendy; C Alouch | Zendy; Laurent Laroche | Zendy; C. Chaumeil | Zendy

Open Access

New tool for the simultaneous detection of 10 different genotypes of Acanthamoeba available from the American Type Culture Collection

Author(s) -

P. Goldschmidt,

Sandrine Degorge,

Djida Ghoubay,

C Saint-Jean,

L. Batellier,

C Alouch,

Laurent Laroche,

C. Chaumeil

Publication year - 2009

Publication title -

british journal of ophthalmology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.016

H-Index - 153

eISSN - 1468-2079

pISSN - 0007-1161

DOI - 10.1136/bjo.2008.144873

Subject(s) - acanthamoeba , taqman , acanthamoeba keratitis , polymerase chain reaction , real time polymerase chain reaction , microbiology and biotechnology , medicine , dna extraction , genotype , biology , virology , gene , genetics

Acanthamoeba keratitis (AK) is a sight-threatening infection, and none of the current diagnosis tests are able to detect in one reaction low levels of the vast majority of strains associated with pathology. The goal of this work was to validate a new tool for the detection of the American Type Cell Collection (ATCC) referenced Acanthamoeba monitoring simultaneously DNA extraction yields and PCR inhibitors. Performances were assessed on corneal scrapings.

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