Open AccessIdentification of Novel Mutations in the N Gene of SARS-CoV-2 That Adversely Affect the Detection of the Virus by Reverse Transcription-Quantitative PCR
Author(s) -
Rashedul Hasan,
Mohammad Enayet Hossain,
Mojnu Miah,
Mohammed Imrul Hasan,
Mustafizur Rahman,
Mohammed Ziaur Rahman
Publication year - 2021
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.502
H-Index - 51
ISSN - 2165-0497
DOI - 10.1128/spectrum.00545-21
Subject(s) - covid-19 , biology , reverse transcriptase , gene , primer (cosmetics) , virus , virology , identification (biology) , genetics , reverse transcription polymerase chain reaction , polymerase chain reaction , computational biology , gene expression , medicine , infectious disease (medical specialty) , chemistry , botany , disease , organic chemistry , pathology , outbreak
Accurate and timely diagnosis of SARS-CoV-2 is a critical step toward controlling the viral spread, since it facilitates the identification and isolation of infected individuals. Mutations in the primer-/probe-binding sites may lead to false-negative results.