Open AccessSingle-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials
Author(s) -
Qingshi Meng,
Liang Chen,
Bohui Xiong,
Beining Kang,
Pengfei Zhang,
Shouwei Tang,
Hui Han,
Wei Shen,
Xiaohui Feng,
Shile Feng,
Ruqing Zhong,
Xiangfang Tang,
Sheng Zhang,
Hongfu Zhang,
Yong Zhao
Publication year - 2021
Publication title -
msystems
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.931
H-Index - 39
ISSN - 2379-5077
DOI - 10.1128/msystems.00725-21
Subject(s) - enterocyte , biology , transcriptome , microbiology and biotechnology , paneth cell , proteomics , receptor , signal transduction , cellular differentiation , ileum , immunology , small intestine , endocrinology , genetics , gene expression , gene
The neonatal period is a crucial time during development of the mammalian small intestine. Moreover, neonatal development and maturation of the small intestine are exceptionally important for early growth, successful weaning, and postweaning growth and development, in order to achieve species-specific milestones. Although several publications recently characterized intestinal epithelial cell diversity at the single-cell level, it remains unclear how differentiation and molecular interactions take place between types and subtypes of epithelial cells during the neonatal period. A single-cell RNA sequencing (scRNA-seq) survey of 40,186 ileal epithelial cells and proteomics analysis of ileal samples at 6 time points in the swine neonatal period were performed. The results revealed previously unknown developmental changes: specific increases in undifferentiated cells, unique enterocyte differentiation, and time-dependent reduction in secretory cells. Moreover, we observed specific transcriptional factors, ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and gut mucosal microbiota playing vital roles in ileal development during the neonatal window. This work offers new comprehensive information regarding ileal development throughout the neonatal period. Reference to this data set may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies. IMPORTANCE We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process. Our results may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies.