Open AccessIsolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.
Author(s) -
George J. Sorger,
David P. Brown,
M Farzannejad,
Alfredo J. Guerra,
Michael Jonathan,
Susan C. Knight,
R Sharda
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.9.4113
Subject(s) - neurospora crassa , biology , nitrogen assimilation , gene , cosmid , glutamine synthetase , regulator gene , gene product , biochemistry , glutamine , assimilation (phonology) , regulation of gene expression , genetics , gene expression , amino acid , linguistics , philosophy , mutant
Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.