Open AccessTranscription factor Sp1 binds to and activates a human hsp70 gene promoter.
Author(s) -
W. T. J. Morgan
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.9.4099
Subject(s) - biology , tata box , caat box , promoter , microbiology and biotechnology , transcription factor , upstream activating sequence , response element , dna footprinting , sp1 transcription factor , e box , transcription (linguistics) , sp3 transcription factor , footprinting , binding site , general transcription factor , gene , gene expression , genetics , linguistics , philosophy
I investigated the binding of purified transcription factor Sp1 from HeLa cells to the human hsp70 promoter by DNase I footprinting. Three binding sites were detected within the upstream promoter region, including one located 46 base pairs upstream of the transcription start, between the TATA box and the proximal CCAAT box element. In vitro transcription demonstrated that the proximal site is capable of responding to Sp1-dependent stimulation. These results suggest that Sp1 might contribute to constitutive expression in vivo and might also be involved in the various regulatory responses that affect this gene.