Open AccessMutation mapping of the 2B5 ecdysone locus in Drosophila melanogaster reveals a long-distance controlling element.
Author(s) -
Javier Sampedro,
Juan Galceran,
Marta López Izquierdo
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.8.3588
Subject(s) - biology , drosophila melanogaster , genetics , locus (genetics) , ecdysone , intron , transcription (linguistics) , gene , transposable element , drosophilidae , p element , mutant , linguistics , philosophy
Mutations at the Broad-Complex, a key gene triggering metamorphosis in Drosophila melanogaster, were mapped at the DNA level. The Broad-Complex includes two mutually complementing allelic classes, br and l(1)2Bc, both of which can associate to insertions at the same intron. Alterations spreading over 50 kilobases upstream from the transcription unit also disrupt br but not l(1)2Bc function.