Open AccessCharacterization and expression of a spliced leader RNA in the parasitic nematode Ascaris lumbricoides var. suum.
Author(s) -
Timothy W. Nilsen,
J Shambaugh,
John A. Denker,
G Chubb,
C Faser,
L Putnam,
Karen Bennett
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.8.3543
Subject(s) - biology , ascaris suum , brugia malayi , rna , nematode , caenorhabditis elegans , ascaris , microbiology and biotechnology , ascaris lumbricoides , genetics , gene , helminths , filariasis , zoology , ecology
The parasitic nematode Ascaris spp. contains a 22-nucleotide spliced-leader (SL) sequence identical to the trans-SL previously described in Caenorhabditis elegans and other nematodes. The SL comprises the first 22 nucleotides of a approximately 110-base RNA and is transcribed by RNA polymerase II. The SL RNA contains a trimethylguanosine cap and a consensus Sm binding site. Furthermore, the Ascaris SL RNA has the potential to adopt a secondary structure which is nearly identical to potential secondary structures of similar SL RNAs in C. elegans and Brugia malayi.