Open AccessTranscription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae.
Author(s) -
Sue E. Stewart,
G. Shirleen Roeder
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.8.3464
Subject(s) - biology , mitotic crossover , transcription (linguistics) , flp frt recombination , rna polymerase i , rna polymerase iii , rna polymerase ii , saccharomyces cerevisiae , genetics , microbiology and biotechnology , transcription bubble , transcription factor ii d , general transcription factor , polymerase , recombination , dna , promoter , genetic recombination , rna dependent rna polymerase , gene , gene expression , linguistics , philosophy
The recombination-stimulating sequence HOT1 is derived from the ribosomal DNA array of Saccharomyces cerevisiae and corresponds to sequences that promote transcription by RNA polymerase I. When inserted at a chromosomal location outside the ribosomal DNA array, HOT1 stimulates mitotic recombination in the adjacent sequences. To investigate the relationship between transcription and recombination, transcription promoted by HOT1 was directly examined. These studies demonstrated that transcription starts at the RNA polymerase I initiation site in HOT1 and proceeds through the chromosomal sequences in which recombination is enhanced. Linker insertion mutations in HOT1 were generated and assayed for recombination stimulation and for promoter function; this analysis demonstrated that the same sequences are required for both activities. These results indicate that the ability of HOT1 to enhance recombination is related to, and most likely dependent on, its ability to promote transcription.