Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers. | Zendy

Dorothy Shippen-Lentz | Zendy; Elizabeth H. Blackburn | Zendy

Open Access

Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers.

Author(s) -

Dorothy Shippen-Lentz,

Elizabeth H. Blackburn

Publication year - 1989

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.9.6.2761

Subject(s) - biology , telomere , tandem repeat , genetics , oligonucleotide , microbiology and biotechnology , rnase p , long terminal repeat , dna , gene , genome , rna

A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3'-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.

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