Open AccessKluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals.
Author(s) -
James O. Deshler,
G P Larson,
J J Rossi
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.5.2208
Subject(s) - kluyveromyces lactis , intron , biology , rna splicing , saccharomyces cerevisiae , kluyveromyces , genetics , gene , exon , yeast , rna
The actin (ACT) gene from the budding yeast Kluyveromyces lactis was cloned, and the nucleotide sequence was determined. The gene had a single intron 778 nucleotides in length which possessed the highly conserved splicing signals found in Saccharomyces cerevisiae introns. We demonstrated splicing of heterologous ACT transcripts in both K. lactis and S. cerevisiae.
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