Open AccessBasal-level expression of the yeast HSP82 gene requires a heat shock regulatory element.
Author(s) -
David H. McDaniel,
Avrom J. Caplan,
MyeongSok Lee,
Christopher C. Adams,
Barbara R. Fishel,
David S. Gross,
William T. Garrard
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.11.4789
Subject(s) - heat shock factor , biology , hsf1 , hspa12a , saccharomyces cerevisiae , transcription factor , heat shock protein , hspa14 , tata box , microbiology and biotechnology , promoter , heat shock , chromatin , hspa4 , transcription (linguistics) , yeast , gene , genetics , gene expression , hsp70 , linguistics , philosophy
Previous studies have shown that heat shock factor is constitutively bound to heat shock elements in Saccharomyces cerevisiae. We demonstrate that mutation of the heat shock element closest to the TATA box of the yeast HSP82 promoter abolishes basal-level transcription without markedly affecting inducibility. The mutated heat shock element no longer bound putative heat shock factor, either in vitro or in vivo, but still resided within a nuclease-hypersensitive site in the chromatin. Thus, constitutive binding of heat shock factor to heat shock elements in S. cerevisiae appears to functionally direct basal-level transcription.