Identification of a new promoter upstream of the murine dihydrofolate reductase gene. | Zendy

Lynda J. Schilling | Zendy; Peggy Farnham | Zendy

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Identification of a new promoter upstream of the murine dihydrofolate reductase gene.

Author(s) -

Lynda J. Schilling,

Peggy Farnham

Publication year - 1989

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.9.10.4568

Subject(s) - dihydrofolate reductase , biology , transcription (linguistics) , microbiology and biotechnology , promoter , messenger rna , abortive initiation , gene , gene expression , genetics , linguistics , philosophy

In vitro reactions identified a transcription initiation site located 740 nucleotides upstream of the dihydrofolate reductase translational start. Transcription from this site proceeded in the direction opposite to that of dihydrofolate reductase mRNA. Deletion mapping indicated that this new promoter can be separated from the dihydrofolate reductase promoter and that separation increased transcription at -740. Transcripts that initiate at -740 were also detected in cellular RNA, indicating that this is a bona fide transcription initiation site in vivo.

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