Open AccessInsertional activation of N-myc by endogenous Moloney-like murine retrovirus sequences in macrophage cell lines derived from myeloma cell line-macrophage hybrids.
Author(s) -
Mihoko Setoguchi,
Yasunori Higuchi,
Seiji Yoshida,
Nobuyoshi Nasu,
Yoshitaka Miyazaki,
Shin’ichiro Akizuki,
Shunsuke Yamamoto
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.10.4515
Subject(s) - biology , retrovirus , murine leukemia virus , macrophage , insertional mutagenesis , cell culture , microbiology and biotechnology , endogenous retrovirus , haematopoiesis , cancer research , virology , genetics , gene , stem cell , genome , in vitro
Hybrids formed from a myeloma cell line, NS1, and macrophages initially show myeloma properties but later, after loss of the parental macrophage genome and consequent loss of myeloma characteristics, express macrophage properties. Molecular studies demonstrated that macrophage properties in the hybridomas originate from the NS1 parental cells (M. Setoguchi, S. Yoshida, Y. Higuchi, S. Akizuki, and S. Yamamoto, Somatic Cell Mol. Genet. 14:427-438, 1988). In such hybrids, N-myc was activated by insertion of endogenous Moloney-like retrovirus sequences into mouse N-myc exon 3 when the hybrids gained macrophage properties. Interestingly, expression of N-myc took place in all aged hybrids. These results suggest that such unique insertional mutagenesis occurs in a regionally specific manner and that expression of N-myc may play a role in hematopoietic lineage conversion.