Open AccessA synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
Author(s) -
Mark Winey,
I Edelman,
Michael R. Culbertson
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.1.329
Subject(s) - biology , intron , transfer rna , rna splicing , gene , saccharomyces cerevisiae , genetics , yeast , rna
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.
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