Open AccessConstruction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance.
Author(s) -
Heidi Stuhlmann,
Rudolf Jaenisch,
Richard C. Mulligan
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.1.100
Subject(s) - biology , murine leukemia virus , virology , insert (composites) , virus , viral replication , dihydrofolate reductase , complementary dna , viral vector , retrovirus , vector (molecular biology) , microbiology and biotechnology , recombinant dna , gene , genetics , mechanical engineering , engineering
A series of replication-competent Moloney murine leukemia virus vectors was constructed in which each vector contained a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat. Two of the resulting viruses, MLV (murine leukemia virus) DHFR*-5 and MLV DHFR*-7, were able to stably transfer methotrexate resistance to infected fibroblast cells upon multiple rounds of virus replication and in the absence of drug selection. Cell lines producing recombinant virus with high titers were established, which indicated that the insert did not grossly interfere with viral replication functions. These vectors should be useful for introducing and expressing foreign genes in vivo in tissues and whole animals in which virus spread is needed for efficient infection.