Open AccessAssembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates.
Author(s) -
Joseph A. Knezetic,
George Adolphus Jacob,
Donal S. Luse
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.8.3114
Subject(s) - transcription factor ii f , transcription preinitiation complex , transcription factor ii e , biology , nucleosome , rna polymerase ii , polymerase , transcription bubble , transcription factor ii a , microbiology and biotechnology , transcription factor ii d , chromatin , dna , genetics , promoter , rna dependent rna polymerase , gene expression , gene
We have previously shown that assembly of nucleosomes on the DNA template blocks transcription initiation by RNA polymerase II in vitro. In the studies reported here, we demonstrate that assembly of a complete RNA polymerase II preinitiation complex before nucleosome assembly results in nucleosomal templates which support initiation in vitro as efficiently as naked DNA. Control experiments prove that our observations are not the result of slow displacement of nucleosomes by the transcription machinery during chromatin assembly, nor are they an artifact of inefficient nucleosome deposition on templates already bearing an RNA polymerase. Thus, the RNA polymerase II preinitiation complex appears to be resistant to disruption by subsequent nucleosome assembly.