Open AccessIndependent transcriptional regulation of a single VL30 element by epidermal growth factor and activators of protein kinase C.
Author(s) -
Karin Rodland,
Leslie L. Muldoon,
Thanh-Hoai Dinh,
Bruce E. Magun
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.5.2247
Subject(s) - protein kinase c , biology , 12 o tetradecanoylphorbol 13 acetate , epidermal growth factor , tetradecanoylphorbol acetate , microbiology and biotechnology , transcription factor , protein kinase a , kinase , cell culture , biochemistry , phorbol ester , genetics , gene
A single VL30 element present in the RVL-3 cell line was transcriptionally induced by both epidermal growth factor (EGF) and the protein kinase C (pkC) activators 12-O-tetradecanoylphorbol-13-acetate (TPA) and sn-1,2-dioctanoylglycerol within 5 min of stimulation. Following TPA-induced depletion of protein kinase C activity, EGF stimulation of VL30 transcription and accumulation was unaffected while TPA effects were inhibited, implying that EGF and TPA act by separable pathways.