p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes. | Zendy

Bret M. Benton | Zendy; Soledad Berríos | Zendy; Paul A. Fisher | Zendy

Open Access

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes.

Author(s) -

Bret M. Benton,

Soledad Berríos,

Paul A. Fisher

Publication year - 1988

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.8.5.1877

Subject(s) - polytene chromosome , biology , drosophila melanogaster , salivary gland , schneider 2 cells , mitosis , interphase , drosophilidae , melanogaster , microbiology and biotechnology , nuclear protein , genetics , gene , transcription factor , rna , biochemistry , rna interference

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

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