Open AccessDNA-binding site for two skeletal actin promoter factors is important for expression in muscle cells.
Author(s) -
Kenneth Walsh,
Paul Schimmel
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.4.1800
Subject(s) - biology , skeletal muscle , transfection , microbiology and biotechnology , binding site , actin , dna binding site , gene expression , regulatory sequence , dna binding protein , promoter , transcription factor , cell culture , gene , genetics , anatomy
Two nuclear factors bind to the same site in the chicken skeletal actin promoter. Mutations in the footprint sequence which eliminate detectable binding decrease expression in transfected skeletal muscle cells by a factor of 25 to 50 and do not elevate the low expression in nonmuscle cells. These results show that the factor-binding site contributes to the activation of expression in muscle cells and that it alone does not contribute significantly to repress expression in nonmuscle cells.