Open AccessAn electrophoretic karyotype of Neurospora crassa.
Author(s) -
Marc J. Orbach,
Douglas Vollrath,
Ronald W. Davis,
Charles Yanofsky
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.4.1469
Subject(s) - neurospora crassa , biology , schizosaccharomyces pombe , crassa , karyotype , dna , genetics , microbiology and biotechnology , chromosomal translocation , neurospora , chromosome , homogeneous , electrophoresis , gel electrophoresis , gene , saccharomyces cerevisiae , physics , mutant , thermodynamics
A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.