Open AccessThe Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1.
Author(s) -
Jef D. Boeke,
Daniel Eichinger,
Diego H. Castrillón,
Gerald R. Fink
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.4.1432
Subject(s) - biology , transposable element , genetics , saccharomyces cerevisiae , genome , transposition (logic) , gene , tn3 transposon , dna , plasmid , open reading frame , mutation , peptide sequence , linguistics , philosophy
Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.