Open AccessThe major inducible heat shock protein hsp68 is not required for acquisition of thermal resistance in mouse plasmacytoma cell lines.
Author(s) -
L Aujame,
H Firko
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.12.5486
Subject(s) - biology , heat shock protein , cell culture , microbiology and biotechnology , plasmacytoma , hsp70 , virology , myeloma protein , antibody , immunology , gene , biochemistry , multiple myeloma , genetics
In mouse cells, the major inducible heat shock protein is a protein of 68,000 daltons (hsp68). We have previously shown that mouse plasmacytomas do not express hsp68. We have now made use of these natural mutants to assess the contribution of hsp68 to acquired thermotolerance. An endpoint limiting dilution assay was used to quantify cell survival to lethal stresses. Two test plasmacytoma cell lines (C1.18.1 and J558) and an hsp68-positive myeloma, XC1.1/51, used as a control, were examined. All showed recovery when pretreated for 10 min at 44 degrees C 2 h before exposure to otherwise lethal stresses of 1 to 4 h at 43 degrees C. Similar results were obtained with the Friend erythroleukemia line D1B, which we have also shown not to express hsp68. These results indicate that hsp68 is not required for protection against thermal stresses in mouse cells.