Open AccessAmplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein.
Author(s) -
Alan Wells,
J M Bishop,
D Helmeste
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.10.4561
Subject(s) - biology , 5 ht5a receptor , microbiology and biotechnology , epidermal growth factor receptor , receptor , gene , mutation , interleukin 21 receptor , growth factor receptor , epidermal growth factor , cancer research , genetics
The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268. The amplified gene gave rise to abundant quantities of receptor that bound EGF with a high affinity (Kd, 0.35 nM). The binding of ligand failed to elicit cellular DNA synthesis, however, and the receptor was enzymatically inactive. We presume that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function. Characterization of the mutation(s) may illuminate how structure dictates function in the receptor protein.