Open AccessPhosphorylation of p53 in normal and simian virus 40-transformed NIH 3T3 cells.
Author(s) -
David W. Meek,
Walter Eckhart
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.1.461
Subject(s) - 3t3 cells , biology , phosphorylation , simian , virus , microbiology and biotechnology , cell culture , virology , transfection , genetics
We observed six major tryptic phosphopeptides in p53 from simian virus 40-transformed and normal NIH 3T3 cells. Analyses of the phosphopeptides indicated that serines 37, 310 and/or 312, 389 and one or more of serines 7, 9, 12, 18, and 23 were phosphorylated. Phosphorylation of serines 310 and/or 312 was twofold higher in the simian virus 40-transformed cells as compared with that in normal NIH 3T3 cells.