Open AccessSubnuclear associations of the v-myb oncogene product and actin are dependent on ionic strength during nuclear isolation.
Author(s) -
W J Boyle,
M A Baluda
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.9.3345
Subject(s) - biology , myb , nuclear matrix , cell nucleus , actin , nuclear protein , microbiology and biotechnology , biochemistry , nucleus , transcription factor , dna , gene , chromatin
The method used to isolate nuclei has a direct effect on the subnuclear association of the v-myb product, p48v-myb, and nuclear actin. Analysis of nuclei subjected to various isolation procedures showed that disruption of native nuclear structure during hypotonic treatment resulted in dissociation of p48v-myb from the nuclear matrix.