Open AccessExtinction of alpha-fetoprotein gene expression in somatic cell hybrids involves cis-acting DNA elements.
Author(s) -
Steven G. Widen,
John Papaconstantinou
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.7.2606
Subject(s) - biology , somatic cell , microbiology and biotechnology , somatic fusion , gene , cell fusion , enhancer , gene expression , dna , fibroblast , cell , genetics , cell culture
alpha-Fetoprotein (AFP), a liver-specific protein, is extinguished in somatic cell hybrids formed by the fusion of mouse hepatoma cells (BWTG3) with rat fibroblast cells (JF1). Our studies show that the extinction of mouse AFP expression in these somatic cell hybrids may involve at least two cis-acting regulatory domains, i.e., the enhancer elements and a tissue-specific promoter region, which are located in the 5'-flanking region of the AFP gene.