Open AccessIdentification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells.
Author(s) -
Nancy Quintrell,
Roger V. Lebo,
Harold Varmus,
J. Michael Bishop,
MJ Pettenati,
Michelle M. Le Beau,
Manuel O. Dı́az,
Janet D. Rowley
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.6.2267
Subject(s) - biology , tyrosine kinase , haematopoiesis , runx1t1 , microbiology and biotechnology , gene , myeloid , myeloproliferative disorders , myeloid leukemia , cancer research , gene expression , signal transduction , genetics , stem cell , immunology
We have isolated cDNAs representing a previously unrecognized human gene that apparently encodes a protein-tyrosine kinase. We have designated the gene as HCK (hemopoietic cell kinase) because its expression is prominent in the lymphoid and myeloid lineages of hemopoiesis. Expression in granulocytic and monocytic leukemia cells increases after the cells have been induced to differentiate. The 57-kilodalton protein encoded by HCK resembles the product of the proto-oncogene c-src and is therefore likely to be a peripheral membrane protein. HCK is located on human chromosome 20 at bands q11-12, a region that is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders. Our findings add to the diversity of protein-tyrosine kinases that may serve specialized functions in hemopoietic cells, and they raise the possibility that damage to HCK may contribute to the pathogenesis of some human leukemias.