Open AccessChromatin structure of the HLA-DR alpha gene in different functional states of major histocompatibility complex class II gene expression.
Author(s) -
B. Matija Peterlin,
K J Hardy,
A S Larsen
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.5.1967
Subject(s) - biology , hypersensitive site , dnase i hypersensitive site , chromatin , gene , microbiology and biotechnology , intron , genetics , deoxyribonuclease i , enhancer , chia pet , human leukocyte antigen , major histocompatibility complex , dna , promoter , gene expression , nucleosome , antigen , base sequence
We utilized DNase I hypersensitivity mapping to study chromatin structure within the HLA-DR alpha gene. We found a single DNase I-hypersensitive site coinciding with the HLA-DR alpha gene promoter in all cells studied. Moreover, in cells that constitutively express HLA-DR, two additional DNase I-hypersensitive sites were observed. These lie within the first intron of the HLA-DR alpha gene and encompass DNA sequences that share homologies with regulatory loci of the immunoglobulin and immune response genes, as well as with core enhancer consensus sequences.