Open AccessFactor interactions at simian virus 40 GC-box promoter elements in intact nuclei.
Author(s) -
Robert L. Buchanan,
Jay D. Gralla
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.4.1554
Subject(s) - biology , primer extension , footprinting , transcription (linguistics) , transcription factor , chromatin , promoter , virus , microbiology and biotechnology , caat box , primer (cosmetics) , gene , genetics , gene expression , base sequence , physics , linguistics , philosophy , thermodynamics
Primer extension footprinting was used to probe late simian virus 40 regulatory elements in intact infected cell nuclei. Specific protection was observed over the viral "GC-box" transcription elements. The participation of the bound templates in gene activation is addressed by quantitation that shows that their abundance greatly exceeds that of transcription complexes but is comparable to that of open chromatin.