Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes. | Zendy

Mark Labow | Zendy; L H Graf | Zendy; Kenneth I. Berns | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Author(s) -

Mark Labow,

L H Graf,

Kenneth I. Berns

Publication year - 1987

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.7.4.1320

Subject(s) - biology , gene , transfection , adeno associated virus , microbiology and biotechnology , genome , selectable marker , transformation (genetics) , dna , gene expression , heterologous , genetics , vector (molecular biology) , recombinant dna

In this paper we report that adeno-associated virus (AAV) genomes inhibit stable transformation by several dominant selectable marker genes upon cotransfection into mouse tissue culture cells. Cotransfection of AAV genomes also inhibited the expression of pSV2cat in transient assays. In both cases, the inhibitory effect was independent of AAV DNA replication but required the AAV p5 and p19 genes, which encode proteins required for AAV DNA replication and regulation of AAV gene expression. Finally, addition of a cloned E4 gene in the transfection experiments partially blocked the AAV-mediated inhibitory activities.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research