Open AccessHuman recipient cell for oncogene transfection studies.
Author(s) -
Michael A. Tainsky,
Fay L. Shamanski,
D. G. Blair,
GF Vande Woude
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.3.1280
Subject(s) - transfection , biology , oncogene , cell culture , 3t3 cells , microbiology and biotechnology , clone (java method) , dna , osteosarcoma , cancer research , cell , cell growth , genetics , cell cycle
We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.