Open AccessExpression of hamster P-glycoprotein and multidrug resistance in DNA-mediated transformants of mouse LTA cells.
Author(s) -
Kathryn L. Deuchars,
Run-Pan Du,
Monisha Naik,
Deanna Evernden-Porelle,
Norbert Kartner,
Alexander M. van der Bliek,
Victor Ling
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.2.718
Subject(s) - hamster , biology , p glycoprotein , multiple drug resistance , gene , microbiology and biotechnology , glycoprotein , transfection , phenotype , chinese hamster ovary cell , dna , chinese hamster , cell culture , membrane glycoproteins , drug resistance , genetics
The overexpression of a plasma membrane glycoprotein, P-glycoprotein, is strongly correlated with the expression of multidrug resistance. This phenotype (frequently observed in cell lines selected for resistance to a single drug) is characterized by cross resistance to many drugs, some of which are used in cancer chemotherapy. In the present study we showed that DNA-mediated transformants of mouse LTA cells with DNA from multidrug-resistant hamster cells acquired the multidrug resistance phenotype, that the transformants contained hamster P-glycoprotein DNA sequences, that these sequences were amplified whereas the recipient mouse P-glycoprotein sequences remained at wild-type levels, and that the overexpressed P-glycoprotein in these cells was of hamster origin. Furthermore, we showed that the hamster P-glycoprotein sequences were transfected independently of a group of genes that were originally coamplified and linked within a 1-megabase-pair region in the donor hamster genome. These data indicate that the high expression of P-glycoprotein is the only alteration required to mediate multidrug resistance.