Open AccessGrowth factor-deprived BALB/c 3T3 murine fibroblasts can enter the S phase after induction of c-myc gene expression.
Author(s) -
F Cavalieri,
Mitchell Goldfarb
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.10.3554
Subject(s) - biology , 3t3 cells , transfection , fgf1 , microbiology and biotechnology , fibroblast growth factor , growth factor , gene expression , platelet derived growth factor receptor , platelet derived growth factor , dna synthesis , cell growth , fgf10 , cell culture , gene , dna , genetics , fibroblast growth factor receptor , receptor
Induction of quiescent BALB/c 3T3 murine fibroblasts by platelet-derived growth factor (PDGF) or fibroblast growth factor (FGFs) is accompanied by induction of c-myc gene expression. To study the role of c-myc in cell growth, we transfected BALB/c 3T3 cells with a plasmid construct containing a glucocorticoid-inducible c-myc gene. When these transfected cells were growth arrested in PDGF-FGF-freedefined medium, glucocorticoid treatment induced S-phase DNA synthesis. This induction of DNA synthesis was inefficient, and cell proliferation was not evident, suggesting that growth factors act through stimulation of c-myc expression together with other intracellular events.